Bio-Rad CFX Opus 96 Dx Real-Time PCR
| Bio-Rad CFX Opus 96 Dx Real-Time PCR | |
|---|---|
| Location: | Lab. 1.84 Building 7 |
| Manager: | Rodrigo Borges |
| Operator: | User |
| Analytical Principle: | Polymerase Chain Reaction |
| Manufacturer: | Bio-Rad Laboratories, Inc. |
| Model: | CFX Opus 96 Dx |
| Type: | Real-Time PCR |
| Website: | Bio-Rad Laboratories, Inc. |
Bio-Rad CFX Opus 96 Dx real-time PCR system, designed for the amplification and quantitative detection of nucleic acid sequences using real-time PCR (qPCR). The equipment features a 96-well reaction block, allowing simultaneous analysis of multiple samples and the performance of quantitative assays, gene expression analysis, melting curve analysis, and allele discrimination.
The Dx version was developed for regulated laboratory environments and includes features focused on data security and traceability, making it suitable for molecular diagnostic applications and the development and validation of real-time PCR methods.
Calendar / Availability
Schedule UseMain Applications:
- Nucleic acid quantification by qPCR
- Genic expression analysis by RT-qPCR
- Melting curves analysis
- Multiplex qPCR assays
- Allelic discrimination
Sample Types:
- Genomic DNA
- Complementary DNA (cDNA) obtained from RNA by reverse transcription (RT-qPCR)
- DNA/cDNA from microorganisms, including bacteria and fungi
- DNA/cDNA extracted from cells and tissues
- DNA/cDNA extracted from environmental samples, including water and sediments
- DNA/cDNA extracted from food and other biological matrices;
- Plasmid DNA and other DNA templates
- Purified nucleic acid preparations
Main Advantages:
- Simultaneous processing of up to 96 samples
- Fluorescence detection across 6 optic channels
- Multiplex detection of up to five targets per well
- High sensitivity and broad dynamic range for quantification
- Flexible thermal cycling for assay optimization
- High thermal uniformity and temperature accuracy.
Technical Specifications:
| Specification | Value |
|---|---|
| Manufacturer | Bio-Rad Laboratories, Inc. |
| Model | CFX Opus 96 Dx |
| Software | CF Maestro DX SE |
| Capacity | 96 wells |
| Sample Volume | 1-50 µL (10–50 µL recommended) |
| Heating/Cooling Method | Peltier |
| Temperature Range | 4-100 °C |
| Maximum Temperature Ramp Speed | 5 °C/s |
| Thermal Precision | ±0,2 °C |
| Optic Detector | 6 LEDs + 6 filtered photodiodes |
| Spectral Range | 450-730 nm |
| Multiplex | Up to 5 targets/well |
| Reading Time | 12s (all channels); 3s (single channel) |
| Interface | Adjustable touch screen |